The bar indicates 100 m. of cell migration and invasion in breast malignancy, the phosphorylation status of this residue may serve as an indication of aggressiveness of breast tumors. gene copy numbers, together with other genes such as and in an amplification stretch in chromosome 1q, are increased in a large percentage of tumors . In glioblastoma multiforme, copy number amplifications in chromosome 19 have been described Chlorothricin leading to increased expression of and . In addition, differential expression of and has been explained for lung adenocarcinoma . The regulation of these gene clusters suggests altered phosphoinositide lipid signaling and lipid-regulated trafficking in these cancers. While little is known on the cellular functions of the two other PIP5K1 enzymes, PIP5K1C (PIP5K1) has been shown to be a major regulator of focal adhesion (FA) dynamics . Depletion of PIP5K1C prospects to cytoskeletal changes and severe attachment defects in cells . Altered FA dynamics due to decrease in PIP5K1C activity or expression has been linked to increased cell migration and invasion [6, 7]. PIP5K1C localization to FA is usually negatively-regulated by p35/Cdk5-mediated phosphorylation at S650 ; and PIP5K1C degradation is usually regulated by phosphorylation through p70S6K1 Chlorothricin at threonine 553 and serine 555 , while its lipid kinase activity is usually inhibited after phosphorylation through protein kinase D (PKD) at serine 448 . Users of the PKD family of serine/threonine kinases control multiple functions within cells by phosphorylating a broad spectrum of targets . In breast malignancy all three isoforms (PKD1, PKD2 and PKD3) have been implicated in regulating malignancy cell survival and proliferation during tumor formation [11C14]. However, with respect to cell migration and invasiveness, it was shown that PKD1 blocks these events through multiple mechanisms. These include PKD1-induced changes in the stability of cell-cell contacts [15C17], in focal adhesion dynamics [9, 17], in actin reorganization dynamics [18C20] and in filopodia formation and stabilization Chlorothricin . Additionally, PKD1 has been shown to block epithelial-to-mesenchymal transition (EMT) [22C24], and to mediate changes in the expression of matrix metalloproteinases (MMPs) . Consequently, in breast malignancy, the transition from a less aggressive to a metastatic phenotype is usually characterized by (PKD1) gene promoter methylation and downregulation of PKD1 expression [14, 26]. We here investigated if expression of PIP5K1C or its phosphorylation status at serine 448 can be indicative for invasive breast cancers. Our data suggest that PKD1 expression levels in tumors correlate with PIP5K1C phosphorylation at serine 448, and that the PIP5K1C phosphorylation at this residue may be a predictive marker for progression to an aggressive phenotype. RESULTS The expression level of PIP5K1C is not predictive for breast cancer survival or subtype Alterations in PIP5K1C expression or activity have been linked to increased cell migration and invasion [6, 7]. We used cBioPortal (http://www.cbioportal.org/public-portal/index.do) to analyze three different available datasets, including 2509 breast cancer (BC) samples, 1105 invasive Rabbit Polyclonal to BRS3 breast carcinoma (IBC, BIC) samples, or 216 metastatic breast cancers (MBC) for gene alterations such as amplification, deletion or mutational events in and genes. While we detected gene amplification of is not predictive for breast Chlorothricin cancer survival or subtype(A) Percent alteration frequency (mutations or alterations in expression) of in 3 studies: breast malignancy (BC; n = 2509 samples; ), breast invasive carcinoma (BIC; n = 1105 samples; TCGA) and mutational profiles of metastatic breast cancers (MBC; n=216 samples; ). The analysis was performed using cBioPortal (http://www.cbioportal.org/public-portal/index.do). (B) Relative expression of in breast malignancy cell lines (n=51) grouped into basal or luminal subtypes (left side) or grouped into TN, HER2+ or HR+ subtypes (right side). The analysis was performed using GOBO from Lund University or college (http://co.bmc.lu.se/gobo/). (C) Tissue microarrays with indicated groups of samples were immunohistochemically-stained for PIP5K1C expression. Relative expression was decided and ranked from 0-6 (0 = no expression; 6 = strongest expression). (D) Distant metastases-free survival (DMFS) of breast cancer patients with high or low expression of over time. The analysis was performed with the Kaplan-Meier Plotter (http://kmplot.com/analysis/index.php?p=service&cancer=breast) using.