After thirty minutes of incubation at +37 1C under an atmosphere containing 5 1% CO2, images of the low face from the inserts were acquired on the Biotek Cytation 5 plate-reading microscope, and analyzed with Halo software (Indica Labs), using the CytoNuclear FL module to count the cells that had crossed the membrane

After thirty minutes of incubation at +37 1C under an atmosphere containing 5 1% CO2, images of the low face from the inserts were acquired on the Biotek Cytation 5 plate-reading microscope, and analyzed with Halo software (Indica Labs), using the CytoNuclear FL module to count the cells that had crossed the membrane. Neutrophil activation […]

Instead, and presumably due to flexibility in the linkers between the MIR and the GFP, an icosahedrally-averaged pattern of density is seen with the greatest concentration being over the 2-fold axes where apparent spikes arise from the capsid surface, and at around the 3-fold and 5-fold axes

Instead, and presumably due to flexibility in the linkers between the MIR and the GFP, an icosahedrally-averaged pattern of density is seen with the greatest concentration being over the 2-fold axes where apparent spikes arise from the capsid surface, and at around the 3-fold and 5-fold axes. particles can be produced with unmodified MIRs, or […]

Pritchard C, Carragher L, Aldridge V, Giblett S, Jin H, Foster C, Andreadi C, Kamata T

Pritchard C, Carragher L, Aldridge V, Giblett S, Jin H, Foster C, Andreadi C, Kamata T. and restores drug level of sensitivity in BRAFV600E CRC drug-resistant cells with high Capture1 levels. In addition, Capture1 targeting from the mitochondria-directed HSP90 chaperones inhibitor gamitrinib induces apoptosis and inhibits colony formation in BRAF-driven CRC cells. Therefore, Capture1 is […]

Immunofluorescence microscopy was performed using the following antibodies: CENP-F (sheep, raised against CENP-F aa 1,363C1,640, 1:1,000; a gift from S

Immunofluorescence microscopy was performed using the following antibodies: CENP-F (sheep, raised against CENP-F aa 1,363C1,640, 1:1,000; a gift from S. Plk4 levels in cells that lack centrioles led to the penetrant formation of de novo centrioles that gained the ability to organize microtubules and duplicate. In summary, we uncover a p53-dependent surveillance mechanism that protects […]

Conversely, p-S6 correlated strongly not only with p-Erk1/2 (< 0

Conversely, p-S6 correlated strongly not only with p-Erk1/2 (< 0.001) but also with p-mTOR (= 0.010), whereas p-Erk1/2 expression correlated weakly with p-mTOR (= 0.014), suggesting that Erk1/2 seems to regulate S6 through mTOR. It is striking that an association between p-AKT expression and p-Erk1/2 expression was not observed, although p-AKT and p-Erk1/2 strongly correlated […]