Despite its high -selectivity evidence for the involvement of this receptor , . Less evidence is usually Kdr available on the affinities of nor-BNI and JDTic for non-opioid targets. competitive antagonists are typically effective for only hours or at most days, antagonism can persist for weeks or weeks after nor-BNI, GNTI or JDTic . To account for this irregular timecourse, it was long presumed that these compounds were slowly soaked up and eliminated. Recently, studies possess suggested instead that nor-BNI, GNTI and JDTic activate the enzyme c-Jun N-terminal kinase 1 (JNK1, MAPK8), causing desensitization of -OR that persists long after 20(S)-NotoginsenosideR2 the compounds are eliminated . Therefore, these compounds appear to induce practical antagonism via a noncompetitive mechanism. Short-acting antagonists did not activate JNK1 . Transient effects Surprisingly, despite the extremely 20(S)-NotoginsenosideR2 protracted timecourse of antagonism, additional effects of nor-BNI, GNTI and JDTic are of quick onset and brief duration , . After subcutaneous (s.c.) administration to mice, nor-BNI and GNTI induce scratching that is maximal within 20 moments and lasts less than two hours , . Nor-BNI and JDTic inhibit self-administration of ethanol by rats at 2 hours, but not 24 hours . Nor-BNI also reduces the maximal responding rate to intracranial self-stimulation in rats on the 1st two hours, but not after 24 hours . In mice, GNTI strongly inhibits locomotor activity within 20 moments, but the effect dissipates within three hours . Nor-BNI inhibits locomotor activity in rats on the day of administration, but not the next day . Despite its high -selectivity evidence for the involvement of this receptor , . Less evidence is definitely available on the affinities of nor-BNI and JDTic for non-opioid focuses on. Nor-BNI has been reported to show very low affinity (confirmation that GNTI functions upon this receptor , . An M1 antagonist experienced no effect. However, those results are hard to interpret, for several reasons. Firstly, in earlier reports M1 agonists induced 20(S)-NotoginsenosideR2 scratching, while antagonists inhibited it . Therefore, the reported inhibition of GNTI-induced scratching by an M1 agonist is definitely paradoxical. Second of all, McN-A-343 was given intrathecally (i.t.), while GNTI was injected s.c. . Due to GNTI’s low potency and extremely low central uptake , this would be unlikely to result in a detectable effect on spinal M1-R. Indeed, GNTI induces scratching after s.c. but not i.t. administration , while M1 agonists show the opposite profile , suggesting that any connection would be indirect. Finally, McN-A-343 is definitely poorly selective for M1 receptors 20(S)-NotoginsenosideR2 , so the involvement of other focuses on cannot be ruled out. Collectively, this evidence is more consistent with an indirect, downstream connection than a competitive one between GNTI and McN-A-343 at spinal M1 receptors. Further exploration of this issue would benefit from the use of more selective M1 ligands, administered from the same route as GNTI. Potential functions of peripheral non-opioid focuses on in the transient effects of nor-BNI and GNTI It remains plausible that peripheral M1 receptors may be involved in some transient effects of GNTI. We previously observed maximum unbound GNTI concentrations in plasma of 2 M at a dose of 10 mg/kg, and 8 M at 39 mg/kg . These concentrations would be likely to result in some peripheral M1 antagonism, given is unclear. While it seems plausible that peripheral receptors might influence reactions such as scratching, this seems less likely for.