To use Prior, primers were validated (by examining melt curves and agarose gel electrophoresis) to amplify one products of anticipated size. labeled clearly. elife-71185-fig1-figsupp1-data5.zip (103K) GUID:?768BE6D9-2F66-4D56-A3E6-7524DF7FEEE7 Figure 1figure dietary supplement 1source data 6: Uncropped traditional western blot scan of P4 WT and KO retinal lysate stained with anti-beta-tubulin antibody. Music group matching to beta-tubulin is tagged. elife-71185-fig1-figsupp1-data6.zip (157K) GUID:?FE673C11-B829-4CEA-92B5-8C1B5E92BFA2 Body 2source data 1: Numerical source data for retinal cell type quantification in P4 and P10 (+)-DHMEQ KO and WT retinas. elife-71185-fig2-data1.xlsx (10K) GUID:?11D0F68D-955A-419C-8E74-7BD538131915 Figure 3source data 1: Gene ontology (Move) overrepresentation analysis of genes differentially expressed in KO retinas at E18.5 and P4 timepoints. Numerical supply data for appearance (in accordance with WT amounts) of go for photoreceptor-specific genes in E18.5 and P4 KO retinas. elife-71185-fig3-data1.xlsx (27K) GUID:?E6D30638-2C20-4C43-8BAA-42D053E6A12C Body 3figure supplement 1source data 1: Numerical source data for expression (in accordance with WT) of go for genes in P4 KO retinas using RNA-seq and RT-qPCR. elife-71185-fig3-figsupp1-data1.xlsx (10K) GUID:?6A265FD3-5025-49DE-9C24-7E317EE698F5 Figure 3figure supplement 1source data 2: Numerical source data for quantification of PHH3-positive nuclei in P0 WT and KO retinal sections. elife-71185-fig3-figsupp1-data2.xlsx (9.6K) GUID:?EFE8EB8C-ADF5-442D-B5E3-D3D4E8017AE8 Figure 3figure dietary supplement 2source data 1: Gene ontology (GO) overrepresentation analysis of significantly changed genes in P4 KO retinas. elife-71185-fig3-figsupp2-data1.xlsx (+)-DHMEQ (20K) GUID:?7489C22F-D00A-4BE5-AABB-C169B483FB2E Body 3figure supplement 3source data 1: Gene ontology (GO) overrepresentation analysis of significantly changed genes in E18.5 KO retinas. elife-71185-fig3-figsupp3-data1.xlsx (16K) GUID:?16B89190-F6B4-4DD9-840F-E8190B8FD983 Figure 3figure supplement 4source data 1: Numerical source data for expression (in accordance (+)-DHMEQ with WT) of retinal cell-type-specific genes in E18.5 and P4 KO retinas from RNA-seq dataset. elife-71185-fig3-figsupp4-data1.xlsx (12K) GUID:?7288A8D5-8FC6-475C-BB1D-F12A4195D3E3 Body 3figure supplement 5source data 1: Numerical source data for expression (in accordance with WT) of many synapse-specific genes in E18.5 and P4 KO retinas. elife-71185-fig3-figsupp5-data1.xlsx (10K) GUID:?8B4E53C0-F7CB-47F1-8E23-AD4A9736EE61 Body 4source data 1: Numerical source data for quantification of Caspase-3-positive (AC3+) and Caspase-3-harmful (AC3-) pyknotic cells in WT and KO retinal sections. Numerical supply data for appearance (in accordance with WT) of cell death-related genes in P4 KO retinas. Numerical supply data for comparative plethora of cyclic ADP-ribose (cADPR) in P4 WT and KO retinas. elife-71185-fig4-data1.xlsx (13K) Mouse monoclonal to CD45/CD14 (FITC/PE) GUID:?D76A6AC5-FD9D-4442-A64B-CBBABE57843B Body 4figure dietary supplement 1source data 1: Numerical source data for expression (in accordance with WT) of cell death-related genes in E18.5 KO retinas. Numerical supply data for comparative plethora of cyclic ADP-ribose (cADPR) in E18.5 WT and KO retinas. elife-71185-fig4-figsupp1-data1.xlsx (10K) GUID:?A15E931C-0F62-4723-B511-13639564C4B3 Body 4figure supplement 1source data 2: Uncropped traditional western blot of P4 KO and WT retinal lysate stained with anti-Gasdermin D (GSDMD) antibody. Arrow denotes rings matching to GSDMD. elife-71185-fig4-figsupp1-data2.zip (422K) GUID:?0ECFABFD-D52D-4A55-81EB-3837527E3139 Body 4figure supplement 1source data 3: Uncropped traditional western blot of P4 KO and WT retinal lysate stained with anti-beta tubulin antibody. Arrow denotes rings matching to beta-tubulin. elife-71185-fig4-figsupp1-data3.zip (356K) GUID:?856AD7A6-1588-48AA-925F-B71E186E9045 Body 4figure supplement 1source data 4: Organic western blot scan of P4 WT and KO retinal lysate stained with anti-Gasdermin D (GSDMD) antibody (700 channel). elife-71185-fig4-figsupp1-data4.zip (613K) GUID:?88FB1FAF-174A-4CC7-A17C-0C2ABF0745D1 Body 4figure supplement 1source data 5: Fresh traditional western blot scan of P4 WT and KO retinal lysate stained with anti-beta tubulin antibody (800 route). elife-71185-fig4-figsupp1-data5.zip (360K) GUID:?8CAE698E-775A-46A7-B622-7D9F612BF3A7 Figure 4figure supplement 2source data 1: Numerical source data for expression of cell loss of life pathway genes in P4 WT and KO retinas. elife-71185-fig4-figsupp2-data1.xlsx (21K) GUID:?73ECE4A2-097C-4F98-8B80-D6EE6EB87B83 Figure 5source data 1: Metabolic pathway overrepresentation analysis in significantly changed metabolites in P4 KO retinas. Numerical supply data for plethora (in accordance with WT) of varied metabolites in P4 KO retinas. Numerical source data for expression of glycolysis/TCA pathway enzymes in P4 KO and WT retinas. elife-71185-fig5-data1.xlsx (20K) GUID:?86187970-6D6E-4839-B832-C1AC5830BB1A Body 5source data 2: Numerical source data for E18.5 and P4 LC-MS/MS tests; peak intensity desks for all discovered metabolites in E18.5 and P4 WT and KO retinas..