The media-duplex solution was further coupled with 6 l of siRNA transfection reagent (sc-29528; Santa Cruz) in 100 l of siRNA transfection medium, incubated for 30 minutes at space temperature, combined with 800 l of transfection medium, and placed on freshly washed cells (50% confluent)

The media-duplex solution was further coupled with 6 l of siRNA transfection reagent (sc-29528; Santa Cruz) in 100 l of siRNA transfection medium, incubated for 30 minutes at space temperature, combined with 800 l of transfection medium, and placed on freshly washed cells (50% confluent). EGFR inside a receptor-low but AREG expressing cell collection improved […]