Soluble proteins (FTT1269, FTT1329, FTT1483, WR101, BB_O34, Rv3362, and OTB1104) were recovered from the culture supernatant following centrifugation (16,000?g, 20?min, 4?C), and purified by FPLC (?KTA, GE Healthcare, Pittsburgh, PA) on nickel columns as described28. the intensity and breadth of antigen-specific immune responses in young and aged mice through the upregulation of immunomodulatory genes in […]
Month: March 2025
The cellular distributions of -actinin-4 and CD2AP, both proteins important for podocyte function, were examined by immunofluorescence following 48?h culture with HI plasma from patients and controls (figure 1A,B,D and E show representative immunofluorescence images)
The cellular distributions of -actinin-4 and CD2AP, both proteins important for podocyte function, were examined by immunofluorescence following 48?h culture with HI plasma from patients and controls (figure 1A,B,D and E show representative immunofluorescence images). protein targets of this phosphorylation. Results HI LN plasma did not alter expression or cellular distribution of podocyte-specific proteins but […]